Journal: Stem Cells Translational Medicine
Article Title: Electrical Cell‐Substrate Impedance Spectroscopy Can Monitor Age‐Grouped Human Adipose Stem Cell Variability During Osteogenic Differentiation
doi: 10.5966/sctm.2015-0404
Figure Lengend Snippet: A comparison of α 2 and R b coefficients of age‐grouped superlots tracked in real time. (A): The α 2 parameter of superlots cultured in complete growth medium (CGM) were lower in the young superlot than the middle‐aged or elderly superlot, indicating greater cell spreading in middle‐aged and elderly superlots. (B): In osteogenic differentiation medium (ODM), the α 2 parameter was higher before differentiation, suggesting that cell spreading decreases in late‐stage osteogenic differentiation. α 2 was highly reduced in the young and middle‐aged superlots as culture time in ODM increased, but α 2 in the young superlot decreased only slightly as the human adipose stem cells (hASCs) were cultured in ODM. (C): R b of the elderly superlot was greatly diminished when compared with the young and middle‐aged superlots cultured in CGM, which indicates fewer cell‐to‐cell junctions in elderly hASCs. (D): In ODM, elderly superlots did not establish an R b coefficient during the study, indicating minimal cell‐to‐cell junctions in elderly hASCs. In the young and middle‐aged superlots, R b decreased after long‐term culture in ODM, at approximately day 6 in the middle‐aged and day 11 in the young superlot. This suggests that the number and composition of intercellular junctions are altered in late‐stage osteogenic differentiation. ∗, Delamination of the young superlot in CGM. ∗∗, Point at which middle‐aged and elderly superlots were ended; at this point, they had undergone differentiation and the impedance drop phase.
Article Snippet: Complete growth medium (CGM) (minimal essential medium, α modified [Thermo Fisher Scientific Life Sciences, Waltham, MA, http://www.thermofisher.com ]) supplemented with 10% fetal bovine serum (FBS) (Gemini Bio Products, West Sacramento, CA, Gemini Bio‐Products, West Sacramento), 2 mM l ‐glutamine (Corning Inc., Corning, NY, http://www.corning.com ), and 100 U/ml penicillin and 100 mg/ml streptomycin (penicillin‐streptomycin solution, Corning Inc.) was used to expand hASCs.
Techniques: Comparison, Cell Culture
